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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has actually been noticed in many tumors, which leads to chromosome instability, and thus may well contribute to tumorigenesis. This protein was identified to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a important function in endorsing cell-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been explained. Two further splice variants were being described but comprehensive nucleotide sequence information and facts is just not however out there.

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Blocking peptides are peptides that bind exclusively to the goal antibody and block antibody binding. These peptide commonly consists of the epitope identified via the antibody.

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This information outlines even further doable optimization of cellular and immunoassay parameters to make certain the very best outcomes are...

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This information outlines even further probable optimization of mobile and immunoassay parameters to be sure the very best effects are...

Antibodies bound to the blocking peptide not bind to your epitope over the target protein. By evaluating the staining from the blocked antibody vs . the antibody by yourself, one can see which staining is particular.

Concentrate on Info Cyclin E belongs into the extremely conserved cyclin spouse and children, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic occasion. Cyclin E types a posh with and functions as a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates at the G1-S section boundary and is also degraded as cells progress as a result of S stage.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...

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